TD PCR

Echenle un vistazo. Nosotros usamos una modificacion de este (step down), pero pueden tratar el original
http://bitesizebio.com/2009/07/20/touchdown-pcr-a-primer-and-some-tips/

Una forma integrativa de mantener propiedades troncales?

141: Nature. 2009 Jul 2;460(7251):66-72. Links

Comment in:

Nature. 2009 Jul 2;460(7251):44-5.

Telomerase modulates Wnt signalling by association with target gene chromatin.

Park JI, Venteicher AS, Hong JY, Choi J, Jun S, Shkreli M, Chang W, Meng Z, Cheung P, Ji H, McLaughlin M, Veenstra TD, Nusse R, McCrea PD, Artandi SE.

Department of Medicine, Stanford University School of Medicine, Stanford, California 94305, USA.

Stem cells are controlled, in part, by genetic pathways frequently dysregulated during human tumorigenesis. Either stimulation of Wnt/beta-catenin signalling or overexpression of telomerase is sufficient to activate quiescent epidermal stem cells in vivo, although the mechanisms by which telomerase exerts these effects are not understood. Here we show that telomerase directly modulates Wnt/beta-catenin signalling by serving as a cofactor in a beta-catenin transcriptional complex. The telomerase protein component TERT (telomerase reverse transcriptase) interacts with BRG1 (also called SMARCA4), a SWI/SNF-related chromatin remodelling protein, and activates Wnt-dependent reporters in cultured cells and in vivo. TERT serves an essential role in formation of the anterior-posterior axis in Xenopus laevis embryos, and this defect in Wnt signalling manifests as homeotic transformations in the vertebrae of Tert(-/-) mice. Chromatin immunoprecipitation of the endogenous TERT protein from mouse gastrointestinal tract shows that TERT physically occupies gene promoters of Wnt-dependent genes. These data reveal an unanticipated role for telomerase as a transcriptional modulator of the Wnt/beta-catenin signalling pathway.

MAS AÚN: "The authors¹ found that, in several cell types, TERT is required for expression of Wnt-regulated genes."

DNA-damaged cells communicate with neighbors to let them know they're in trouble.

When cells experiencing DNA damage fail to repair themselves, they send a signal to their neighbors letting them know they're in trouble. The discovery, which shows that a process dubbed the DDR (DNA Damage Response) also controls communication from cell to cell, has implications for both cancer and aging. The findings appear in the July 13 online edition of the Nature Cell Biology.

http://132.248.116.102:2056/ncb/journal/vaop/ncurrent/pdf/ncb1909.pdf